Difference between revisions of "Part:BBa K3726112"
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+ | This part is an homology 5’ connector harboring the up homology arm for the homologous recombination within the Neutral Site Nº 5 of PCC11801. | ||
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+ | Within the Marburg Collection design, connectors are found in the upstream (5’) and downstream (3’) regions of the level 1 assemblies, flanking the transcriptional unit. This allows the further assembly of multiple transcriptional units into a level 2 genetic construct using level 1 parts flanked by different connectors. To know more about, read the design principles of Marburg Collection: https://2019.igem.org/Team:Marburg/Part_Collection. | ||
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+ | Among other functions, connectors can also be used to include homology regions for integrative recombination in the chassis genome. This way 5’ Connector parts can harbor the up / down homology arm required for double homologous recombination. | ||
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+ | The part has been designed in accordance with the Marburg Collection guidelines. 5’ / 3’ Connectors correspond with the parts found Upstream / Downstream of the level 1 assembled transcriptional unit. | ||
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− | <span class='h3bb'>Sequence | + | <span class='h3bb'><b>Sequence & Features</b></span> |
<partinfo>BBa_K3726112 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3726112 SequenceAndFeatures</partinfo> | ||
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<partinfo>BBa_K3726112 parameters</partinfo> | <partinfo>BBa_K3726112 parameters</partinfo> | ||
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+ | The genomic integration loci targeted by this part corresponds with a novel neutral site for Synechococcus PCC11801. This neutral site has been identified by the MADRID_UCM 2021 iGEM team as a part of their project. To know more about the discovery process visit the section Software of 4C_Fuels project. https://2021.igem.org/Team:MADRID_UCM/Software |
Latest revision as of 14:48, 8 October 2021
5CON1(H)_NS5-up (PCC 11801)
This part is an homology 5’ connector harboring the up homology arm for the homologous recombination within the Neutral Site Nº 5 of PCC11801.
Within the Marburg Collection design, connectors are found in the upstream (5’) and downstream (3’) regions of the level 1 assemblies, flanking the transcriptional unit. This allows the further assembly of multiple transcriptional units into a level 2 genetic construct using level 1 parts flanked by different connectors. To know more about, read the design principles of Marburg Collection: https://2019.igem.org/Team:Marburg/Part_Collection.
Among other functions, connectors can also be used to include homology regions for integrative recombination in the chassis genome. This way 5’ Connector parts can harbor the up / down homology arm required for double homologous recombination.
The part has been designed in accordance with the Marburg Collection guidelines. 5’ / 3’ Connectors correspond with the parts found Upstream / Downstream of the level 1 assembled transcriptional unit.
Sequence & Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 72
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 72
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 72
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 72
- 1000COMPATIBLE WITH RFC[1000]
The genomic integration loci targeted by this part corresponds with a novel neutral site for Synechococcus PCC11801. This neutral site has been identified by the MADRID_UCM 2021 iGEM team as a part of their project. To know more about the discovery process visit the section Software of 4C_Fuels project. https://2021.igem.org/Team:MADRID_UCM/Software