Difference between revisions of "Part:BBa K3726095:Design"
 
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===Design Notes===
 
===Design Notes===
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The part is a marburg collection design MoClo Lv.0 part, assembled in the universal phytobrick entry vector BBa_K2560001. The upstream overhang is GCTTAA where AA are extra bases for creating an additional stop codon (TAA) in the end of the upstream coding sequence in accordance with the Marburg Collection design guidelines.  
 
The part is a marburg collection design MoClo Lv.0 part, assembled in the universal phytobrick entry vector BBa_K2560001. The upstream overhang is GCTTAA where AA are extra bases for creating an additional stop codon (TAA) in the end of the upstream coding sequence in accordance with the Marburg Collection design guidelines.  
  
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This terminator part spans the 5 position of the MoClo syntax. The downstream overhang is CGCT. This part behaves like a normal phytobrick standard terminator which in the design of Marburg Collection is connected to a 3’ Connector part during the level 1 MoClo reaction.
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This terminator part spans the 5A position of the MoClo syntax. The downstream overhang is GGTA, allowing the assembly of an additional expression cassette downstream the terminator part.
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===Source===
 
===Source===
  
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xxx
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This part has been artificially designed and synthetized as part of the research of G. Gale, B. Wang and A. McCormick.
  
 
===References===
 
===References===
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xxx
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G. Gale, B. Wang and A. McCormick, "Evaluation and Comparison of the Efficiency of Transcription Terminators in Different Cyanobacterial Species", Frontiers in Microbiology, vol. 11, 2021.

Latest revision as of 17:26, 7 October 2021

T(5a)_Teck


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part is a marburg collection design MoClo Lv.0 part, assembled in the universal phytobrick entry vector BBa_K2560001. The upstream overhang is GCTTAA where AA are extra bases for creating an additional stop codon (TAA) in the end of the upstream coding sequence in accordance with the Marburg Collection design guidelines.

This terminator part spans the 5A position of the MoClo syntax. The downstream overhang is GGTA, allowing the assembly of an additional expression cassette downstream the terminator part.


Source

This part has been artificially designed and synthetized as part of the research of G. Gale, B. Wang and A. McCormick.

References

G. Gale, B. Wang and A. McCormick, "Evaluation and Comparison of the Efficiency of Transcription Terminators in Different Cyanobacterial Species", Frontiers in Microbiology, vol. 11, 2021.