Difference between revisions of "Part:BBa K3726094"
 
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<partinfo>BBa_K3726000 short</partinfo>
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<partinfo>Bba_K3726094 short</partinfo>
  
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This part corresponds with the protein coding sequence for an Acetoacetyl CoA synthase.  
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The sequence of this parts corresponds with a rho-independent (intrinsec) terminator with a proved 99.9% terminating efficiency in UTEX 2973 either in optimal and suboptimal growth conditions.
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The part is a marburg collection design MoClo Lv.0 part, assembled in the universal phytobrick entry vector BBa_K2560001. The upstream overhang is GCTTAA where AA are extra bases for creating an additional stop codon (TAA) in the end of the upstream coding sequence in accordance with the Marburg Collection design guidelines.  
  
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The codified enzyme from <i>Streptomyces sp. CL190</i>.(NphT7) catalyzes the irreversible condensation-decarboxylation step of 1 molecule of acetyl-coA with Malonyl-coA to transform them into Acetoacetyl-coA and CO2.
 
  
 
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<span class='h3bb'><b>Sequence & Features</b></span>
 
<span class='h3bb'><b>Sequence & Features</b></span>
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<partinfo>BBa_K3726000 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K3726094 SequenceAndFeatures</partinfo>
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K3726000 parameters</partinfo>
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<partinfo>BBa_K3726094 parameters</partinfo>
 
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Latest revision as of 17:23, 7 October 2021

T(5a)_Teck

The sequence of this parts corresponds with a rho-independent (intrinsec) terminator with a proved 99.9% terminating efficiency in UTEX 2973 either in optimal and suboptimal growth conditions.

The part is a marburg collection design MoClo Lv.0 part, assembled in the universal phytobrick entry vector BBa_K2560001. The upstream overhang is GCTTAA where AA are extra bases for creating an additional stop codon (TAA) in the end of the upstream coding sequence in accordance with the Marburg Collection design guidelines.


Sequence & Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]