Difference between revisions of "Part:BBa K3809004"

 
 
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<partinfo>BBa_K3809004 short</partinfo>
 
<partinfo>BBa_K3809004 short</partinfo>
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This sequence codes for the enzyme alditol oxidase from the organism Streptomyces coelicolor. It was designed as part of a novel selection method in which the enzyme converts glycerol into D-glycerate. This gene will work alongside PuDHT and katE to develop a new selection mechanism which involves the knockout of genes gldA, glpK and oxyR to make E. coli suceptible to death by exposure to low concentrations of glycerol. We will substitute the genes gldA and glpK with PuDHT and aldO, and transform the bacteria with a plasmid containing selection cassette katE. AldO will convert glycerol to peroxide and D-glyceric acid, katE will convert peroxide to water and oxygen and PuDHT will convert D-glyceric acid into pyruvate.
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This sequence codes for the enzyme alditol oxidase from the organism Streptomyces coelicolor. It was designed as part of a novel selection method in which the enzyme converts glycerol into a useful compound for bacteria, alongside PuDHT and katE.
 
  
 
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Latest revision as of 15:19, 7 October 2021


aldO

This sequence codes for the enzyme alditol oxidase from the organism Streptomyces coelicolor. It was designed as part of a novel selection method in which the enzyme converts glycerol into D-glycerate. This gene will work alongside PuDHT and katE to develop a new selection mechanism which involves the knockout of genes gldA, glpK and oxyR to make E. coli suceptible to death by exposure to low concentrations of glycerol. We will substitute the genes gldA and glpK with PuDHT and aldO, and transform the bacteria with a plasmid containing selection cassette katE. AldO will convert glycerol to peroxide and D-glyceric acid, katE will convert peroxide to water and oxygen and PuDHT will convert D-glyceric acid into pyruvate.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 585
    Illegal BamHI site found at 1203
    Illegal XhoI site found at 287
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 28
  • 1000
    COMPATIBLE WITH RFC[1000]