Difference between revisions of "Part:BBa K3905013"

 
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<partinfo>BBa_K3905013 short</partinfo>
 
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Amplification probe required for the isothermal amplification of the Specified micro-RNA using Recombinase polymerase amplification. To isothermally amplify micro-RNA for practical purposes, required the design of the 517-5 Probe 1.  
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Amplification probe required for the isothermal amplification of miRNA using Recombinase polymerase amplification. RPA of the miRNA required the design of the 517-5 Probe 1. Probe 1 binds to the 5' end of the miRNA. This probe is phosphorylated on its 5' end. Through phosphorylation, DNA ligase is able to ligate 517-5 Probe 1 to 517-5 Probe 2. Allowing RPA to occur for the formation of DNA. For this to occur, the forward primer
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5' AACCAGCCACAAACATAATACGACTC 3' is added, which binds to this probe.
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 10:57, 7 October 2021


517-5 Probe 1

Amplification probe required for the isothermal amplification of miRNA using Recombinase polymerase amplification. RPA of the miRNA required the design of the 517-5 Probe 1. Probe 1 binds to the 5' end of the miRNA. This probe is phosphorylated on its 5' end. Through phosphorylation, DNA ligase is able to ligate 517-5 Probe 1 to 517-5 Probe 2. Allowing RPA to occur for the formation of DNA. For this to occur, the forward primer 5' AACCAGCCACAAACATAATACGACTC 3' is added, which binds to this probe. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]