Difference between revisions of "Part:BBa K3905015"

Revision as of 10:52, 7 October 2021

210-3p Probe 1

Amplification probe required for the isothermal amplification of miRNA using Recombinase polymerase amplification. RPA of the miRNA required the design of the 210-3p Probe 1. Probe 1 binds to the 5' end of the miRNA. This probe is phosphorylated on its 5' end. Through phosphorylation, DNA ligase is able to ligate 210-3p Probe 1 to 210-3p Probe 2. Allowing RPA to occur for the formation of DNA. For this to occur, the forward primer 5' AAACAAGAAACAGGATAATACGACTC 3' is added, which binds to this probe.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3905015 short</partinfo>
-Amplification probe required for the isothermal amplification of miRNA using Recombinase polymerase amplification. RPA of the miRNA required the design of the 517-5 Probe 1. Probe 1 binds to the 5' end of the miRNA. This probe is phosphorylated on its 5' end. Through phosphorylation, DNA ligase is able to ligate 210-3p Probe 1 to 210-3p Probe 2. Allowing RPA to occur for the formation of DNA.
+Amplification probe required for the isothermal amplification of miRNA using Recombinase polymerase amplification. RPA of the miRNA required the design of the 210-3p Probe 1. Probe 1 binds to the 5' end of the miRNA. This probe is phosphorylated on its 5' end. Through phosphorylation, DNA ligase is able to ligate 210-3p Probe 1 to 210-3p Probe 2. Allowing RPA to occur for the formation of DNA.
 For this to occur, the forward primer 5' AAACAAGAAACAGGATAATACGACTC 3' is added, which binds to this probe.