Difference between revisions of "Part:BBa K3726037:Design"

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Codon optimization has been performed using DeNovo DNA Software, adjusting the codon usage for high expression in <i>Synechococcus elongatus PCC7942</i>.
 
Codon optimization has been performed using DeNovo DNA Software, adjusting the codon usage for high expression in <i>Synechococcus elongatus PCC7942</i>.
 
In addition the part sequence has been optimized to improve  mRNA stability removing internal recognition sites for endonucleases
 
In addition the part sequence has been optimized to improve  mRNA stability removing internal recognition sites for endonucleases
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===Source===
 
===Source===
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Coding sequence of this Malyl-CoA lyase has been found within the genome of <i>Rhodobacter sphaeroides</i>.
 
Coding sequence of this Malyl-CoA lyase has been found within the genome of <i>Rhodobacter sphaeroides</i>.
 
Uniprot reference: https://www.uniprot.org/uniprot/Q3J5L6
 
Uniprot reference: https://www.uniprot.org/uniprot/Q3J5L6
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===References===
 
===References===
 
H. Yu, X. Li, F. Duchoud, D. Chuang and J. Liao, "Augmenting the Calvin–Benson–Bassham cycle by a synthetic malyl-CoA-glycerate carbon fixation pathway", 2021.
 
H. Yu, X. Li, F. Duchoud, D. Chuang and J. Liao, "Augmenting the Calvin–Benson–Bassham cycle by a synthetic malyl-CoA-glycerate carbon fixation pathway", 2021.

Latest revision as of 23:36, 6 October 2021


CDS_mcl


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 839
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 839
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 839
    Illegal BamHI site found at 609
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 839
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 839
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part corresponds with the codon optimized coding sequence of mcl enzyme. Codon optimization has been performed using DeNovo DNA Software, adjusting the codon usage for high expression in Synechococcus elongatus PCC7942. In addition the part sequence has been optimized to improve mRNA stability removing internal recognition sites for endonucleases

Source

Coding sequence of this Malyl-CoA lyase has been found within the genome of Rhodobacter sphaeroides. Uniprot reference: https://www.uniprot.org/uniprot/Q3J5L6


References

H. Yu, X. Li, F. Duchoud, D. Chuang and J. Liao, "Augmenting the Calvin–Benson–Bassham cycle by a synthetic malyl-CoA-glycerate carbon fixation pathway", 2021.