Difference between revisions of "Part:BBa K3726025:Design"
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===Design Notes===
 
===Design Notes===
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This part has been designed to be assemble within the part "BBa_K3726028" through Gibson Assembly.
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This part has been designed to be assembled within the part "BBa_K3726028" through Gibson Assembly.
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===Source===
 
===Source===
  
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Coding sequence of this Glycerate 2-kinase has been obtained from <i>Escherichia coli (strain BL21)</i> genome thought PCR.
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Coding sequence of this Glycerate 2-kinase has been obtained from <i>Escherichia coli (strain BL21)</i> genome through PCR.
 
Uniprot reference:https://www.uniprot.org/uniprot/P23524
 
Uniprot reference:https://www.uniprot.org/uniprot/P23524
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===References===
 
===References===

Latest revision as of 23:16, 6 October 2021


CDS_GarK (E. Coli BL21)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 355
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 355
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 355
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 355
    Illegal AgeI site found at 868
    Illegal AgeI site found at 951
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part has been designed to be assembled within the part "BBa_K3726028" through Gibson Assembly.

Source

Coding sequence of this Glycerate 2-kinase has been obtained from Escherichia coli (strain BL21) genome through PCR. Uniprot reference:https://www.uniprot.org/uniprot/P23524

References