Difference between revisions of "Part:BBa K4055537"
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<partinfo>BBa_K4055537 short</partinfo> | <partinfo>BBa_K4055537 short</partinfo> | ||
− | This component paty acts as a blue light sensing system that produces t7RNA polymerase, enhancing gene expression at the back-end of the T7 promoter. | + | This component paty acts as a blue light sensing system that produces t7RNA polymerase, enhancing gene expression at the back-end of the T7 promoter.In fact, there are holes in the system. The system is located on a low copy plasmid, while the plasmid expressing the protein is a high copy plasmid. So the protein can leak out. The system does not achieve what we are looking for |
+ | ===Experiment=== | ||
+ | Wrap it in black paper, cut out a specific pattern and illuminate it with blue light. After a few days the proteins were observed to be expressed in a specific pattern. Unfortunately, the protein was not expressed in sufficient concentrations and leakage occurred | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
<!-- --> | <!-- --> | ||
− | <span class='h3bb'>Sequence and Features</span> | + | <span class='h3bb'><h3>Sequence and Features</h3></span> |
<partinfo>BBa_K4055537 SequenceAndFeatures</partinfo> | <partinfo>BBa_K4055537 SequenceAndFeatures</partinfo> | ||
Latest revision as of 16:13, 6 October 2021
BlueLight_inducing_T7RNAPolymerase
This component paty acts as a blue light sensing system that produces t7RNA polymerase, enhancing gene expression at the back-end of the T7 promoter.In fact, there are holes in the system. The system is located on a low copy plasmid, while the plasmid expressing the protein is a high copy plasmid. So the protein can leak out. The system does not achieve what we are looking for
Experiment
Wrap it in black paper, cut out a specific pattern and illuminate it with blue light. After a few days the proteins were observed to be expressed in a specific pattern. Unfortunately, the protein was not expressed in sufficient concentrations and leakage occurred
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 79
Illegal NgoMIV site found at 372
Illegal NgoMIV site found at 466
Illegal NgoMIV site found at 598
Illegal NgoMIV site found at 703
Illegal NgoMIV site found at 996
Illegal NgoMIV site found at 1090
Illegal AgeI site found at 247
Illegal AgeI site found at 871 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 136
Illegal BsaI site found at 760