Difference between revisions of "Part:BBa K4055524"
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Revision as of 15:19, 6 October 2021
ribozyne-based insulator RiboJ
RiboJ comprises of the sTRSV-ribozyme with anadditional 23-nt hairpin immediately downstream to help expose the RBS. sTRSV autocatalytically cleaved the mRNA at a defined residue, which had the effect of cleaving extraneous RNA leaders that arose from transcription from promoters with different start sites.This can insulate the output from the influence of the genetic context, meaning that the output promoter depended only on the activity of the input promoter parts, and not the sequence at the part junction.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]