Difference between revisions of "Part:BBa K3726095:Design"

 
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__NOTOC__
 
__NOTOC__
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<partinfo>BBa_K3726095 short</partinfo>
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<partinfo>BBa_K3726094 short</partinfo>
  
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<partinfo>BBa_K3726095 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K3726094 SequenceAndFeatures</partinfo>
  
  
 
===Design Notes===
 
===Design Notes===
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The part is a marburg collection design MoClo Lv.0 part, assembled in the universal phytobrick entry vector BBa_K2560001. The upstream overhang is GCTTAA where AA are extra bases for creating an additional stop codon (TAA) in the end of the upstream coding sequence in accordance with the Marburg Collection design guidelines.
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This terminator part spans the 5 position of the MoClo syntax. The downstream overhang is CGCT. This part behaves like a normal phytobrick standard terminator which in the design of Marburg Collection is connected to a 3’ Connector part during the level 1 MoClo reaction.
  
 
===Source===
 
===Source===
  
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xxxbbx
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xxx
  
 
===References===
 
===References===
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xxx

Revision as of 10:59, 6 October 2021

T(5a)_Teck


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part is a marburg collection design MoClo Lv.0 part, assembled in the universal phytobrick entry vector BBa_K2560001. The upstream overhang is GCTTAA where AA are extra bases for creating an additional stop codon (TAA) in the end of the upstream coding sequence in accordance with the Marburg Collection design guidelines.

This terminator part spans the 5 position of the MoClo syntax. The downstream overhang is CGCT. This part behaves like a normal phytobrick standard terminator which in the design of Marburg Collection is connected to a 3’ Connector part during the level 1 MoClo reaction.

Source

xxx

References

xxx