Difference between revisions of "Part:BBa K3889000"

Revision as of 03:39, 6 October 2021

Azurite_BFP (BFP.B3)

Azurite is a Blue Fluorescence Reporter protein from Aequorea victoria and has the following parameters [1,2]:

Ex : Peak excitation wavelength (in λ)	383
Em: Peak emission wavelength (in λ)	447
EC: Extinction Coefficient (M^-1 cm^-1)	26,200
QY: Quantum Yield	0.55
Brightness	14.41
pKa	5.0
Maturation (min)	18.0
Lifetime (ns)	3.65

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Reference

1. Mena, M. A., Treynor, T. P., Mayo, S. L., & Daugherty, P. S. (2006). Blue fluorescent proteins with enhanced brightness and photostability from a structurally targeted library. Nature biotechnology, 24(12), 1569–1571. https://doi.org/10.1038/nbt1264

2. https://www.fpbase.org/protein/azurite/

@@ Line 13: / Line 13: @@
 |447
 |-
-!EC: Extinction Coefficient  (M-1 cm-1)
+!EC: Extinction Coefficient  (M<sup>-1</sup> cm<sup>-1</sup>)
 |26,200
 |-