Difference between revisions of "Part:BBa K3787002"

 
Line 3: Line 3:
 
<partinfo>BBa_K3787002 short</partinfo>
 
<partinfo>BBa_K3787002 short</partinfo>
  
−
This part is a chimeric protein used to depolymerize PET into its constituting monomers. It consists of two enzymes, the mutant PETase S245I and MHETase. The PETase part degrades PET into bis(2-hydroxyethyl) terephthalic acid (BHET), mono(2-hydroxyethyl) terephthalic acid (MHET), and terephthalic acid (TPA), while the MHETase part degrades MHET into TPA and ethylene glycol (EG). The C-terminus of PETase is linked to the N-terminus of MHETase using a 12 amino acid serine-glycine linker. Additionally, the part coding for PETase is genetically modified and optimized for PET digestion. The gene is modified such that the 245th amino acid coded is changed from serine to isoleucine.
+
This part is a chimeric protein used to depolymerize PET into its constituting monomers. It consists of two enzymes, the mutant PETase S245I and MHETase. The PETase part degrades PET into bis(2-hydroxyethyl) terephthalic acid (BHET), mono(2-hydroxyethyl) terephthalic acid (MHET), and terephthalic acid (TPA), while the MHETase part degrades MHET into TPA and ethylene glycol (EG). The C-terminus of PETase is linked to the N-terminus of MHETase using a 12 amino acid serine-glycine linker. Additionally, the part coding for PETase is genetically modified and optimized for PET digestion. The gene is modified such that the 245th amino acid coded is changed from serine to isoleucine. The part has a C-terminus 6xHis-tag for purification.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 21:58, 5 October 2021


S245I PETase-MHETase

This part is a chimeric protein used to depolymerize PET into its constituting monomers. It consists of two enzymes, the mutant PETase S245I and MHETase. The PETase part degrades PET into bis(2-hydroxyethyl) terephthalic acid (BHET), mono(2-hydroxyethyl) terephthalic acid (MHET), and terephthalic acid (TPA), while the MHETase part degrades MHET into TPA and ethylene glycol (EG). The C-terminus of PETase is linked to the N-terminus of MHETase using a 12 amino acid serine-glycine linker. Additionally, the part coding for PETase is genetically modified and optimized for PET digestion. The gene is modified such that the 245th amino acid coded is changed from serine to isoleucine. The part has a C-terminus 6xHis-tag for purification.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 348
    Illegal PstI site found at 1889
    Illegal PstI site found at 1918
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 304
    Illegal NheI site found at 1038
    Illegal PstI site found at 1889
    Illegal PstI site found at 1918
    Illegal NotI site found at 1586
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 348
    Illegal PstI site found at 1889
    Illegal PstI site found at 1918
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 348
    Illegal PstI site found at 1889
    Illegal PstI site found at 1918
    Illegal NgoMIV site found at 1513
    Illegal AgeI site found at 627
  • 1000
    COMPATIBLE WITH RFC[1000]