Difference between revisions of "Part:BBa K3726019:Design"
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===Design Notes=== | ===Design Notes=== | ||
This part corresponds with the codon optimized coding sequence of PtaBs. | This part corresponds with the codon optimized coding sequence of PtaBs. | ||
| − | Codon optimization has been performed using DeNovo DNA Software, adjusting the codon usage for high expression in Synechococcus elongatus PCC7942. | + | Codon optimization has been performed using DeNovo DNA Software, adjusting the codon usage for high expression in <i>Synechococcus elongatus PCC7942</i>. |
In addition the part sequence has been optimized to improve mRNA stability removing internal recognition sites for endonucleases | In addition the part sequence has been optimized to improve mRNA stability removing internal recognition sites for endonucleases | ||
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===Source=== | ===Source=== | ||
Latest revision as of 13:53, 5 October 2021
CDS_PtaBs
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part corresponds with the codon optimized coding sequence of PtaBs. Codon optimization has been performed using DeNovo DNA Software, adjusting the codon usage for high expression in Synechococcus elongatus PCC7942. In addition the part sequence has been optimized to improve mRNA stability removing internal recognition sites for endonucleases
Source
Coding sequence of this Phosphate acetyltransferase has been found within the genome of Bacillus subtilis . Uniprot reference: https://www.uniprot.org/uniprot/P39646
References
[7]"Catabolite Regulation of the pta Gene as Part of Carbon Flow Pathways in Bacillus subtilis | Journal of Bacteriology", Journal of Bacteriology, 2021. [Online]. Available: https://journals.asm.org/doi/10.1128/jb.181.22.6889-6897.1999?permanently=true. [Accessed: 05- Oct- 2021].