Difference between revisions of "Part:BBa K3726019:Design"

 
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===Design Notes===
 
===Design Notes===
 
This part corresponds with the codon optimized coding sequence of PtaBs.
 
This part corresponds with the codon optimized coding sequence of PtaBs.
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Codon optimization has been performed using DeNovo DNA Software, adjusting the codon usage for high expression in Synechococcus elongatus PCC7942.
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Codon optimization has been performed using DeNovo DNA Software, adjusting the codon usage for high expression in <i>Synechococcus elongatus PCC7942</i>.
 
In addition the part sequence has been optimized to improve  mRNA stability removing internal recognition sites for endonucleases
 
In addition the part sequence has been optimized to improve  mRNA stability removing internal recognition sites for endonucleases
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−
 
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===Source===
 
===Source===
  
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Coding sequence of this Phosphate acetyltransferase has been found within the genome of Bacillus subtilis .
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Coding sequence of this Phosphate acetyltransferase has been found within the genome of <i>Bacillus subtilis</i> .
 
Uniprot reference: https://www.uniprot.org/uniprot/P39646
 
Uniprot reference: https://www.uniprot.org/uniprot/P39646
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===References===
 
===References===
 +
[7]"Catabolite Regulation of the pta Gene as Part of Carbon Flow Pathways in <i>Bacillus subtilis</i> | Journal of Bacteriology", Journal of Bacteriology, 2021. [Online]. Available: https://journals.asm.org/doi/10.1128/jb.181.22.6889-6897.1999?permanently=true. [Accessed: 05- Oct- 2021].

Latest revision as of 13:53, 5 October 2021


CDS_PtaBs


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part corresponds with the codon optimized coding sequence of PtaBs. Codon optimization has been performed using DeNovo DNA Software, adjusting the codon usage for high expression in Synechococcus elongatus PCC7942. In addition the part sequence has been optimized to improve mRNA stability removing internal recognition sites for endonucleases

Source

Coding sequence of this Phosphate acetyltransferase has been found within the genome of Bacillus subtilis . Uniprot reference: https://www.uniprot.org/uniprot/P39646

References

[7]"Catabolite Regulation of the pta Gene as Part of Carbon Flow Pathways in Bacillus subtilis | Journal of Bacteriology", Journal of Bacteriology, 2021. [Online]. Available: https://journals.asm.org/doi/10.1128/jb.181.22.6889-6897.1999?permanently=true. [Accessed: 05- Oct- 2021].