Difference between revisions of "Part:BBa K3726001:Design"
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===Design Notes=== | ===Design Notes=== | ||
This part corresponds with the codon optimized coding sequence of PhaB^173S enzyme. | This part corresponds with the codon optimized coding sequence of PhaB^173S enzyme. | ||
| − | Codon optimization has been performed using DeNovo DNA Software, adjusting the codon usage for high expression in Synechococcus elongatus PCC7942. | + | Codon optimization has been performed using DeNovo DNA Software, adjusting the codon usage for high expression in <i>Synechococcus elongatus PCC7942</i>. |
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===Source=== | ===Source=== | ||
Revision as of 13:25, 5 October 2021
CDS_PhaB^173S
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part corresponds with the codon optimized coding sequence of PhaB^173S enzyme. Codon optimization has been performed using DeNovo DNA Software, adjusting the codon usage for high expression in Synechococcus elongatus PCC7942.
Source
Coding sequence of this Acetoacetyl-CoA reductase has been found within the genome of Ralstoni eutropha. Uniprot reference: https://www.uniprot.org/uniprot/P14697
References
"Directed Evolution and Structural Analysis of NADPH-Dependent Acetoacetyl Coenzyme A (Acetoacetyl-CoA) Reductase from Ralstonia eutropha Reveals Two Mutations Responsible for Enhanced Kinetics | Applied and Environmental Microbiology", Applied and Environmental Microbiology, 2021. [Online]. Available: https://journals.asm.org/doi/10.1128/aem.01768-13?permanently=true.