Difference between revisions of "Part:BBa K3882002:Design"

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===Design Notes===
 
===Design Notes===
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T7 promoter is a high efficiency element which can have a high protein level of LUXR inside the BL21 E. coli bacteria. High level of LUXR expressed will makes the BL21 more sensitive to AHL in the environment.
  
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Lac operator is an inhibitory DNA element which can shut down the function of AHL sensing by inhibit the transcription of LuxR gene. Thus, the AHL release by the BL21 E. coli can not be detected when culturing at the initial steps.
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pelB is a commonly used signaling peptide for locating the protein into periplasm.
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pvdq is the most widely used gene in the quorum system. To block quorum system activation, we use the PVDQ protein to clean up all the AHL in the environment released by P. aeruginosa.
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protein linker is a commonly used in the fused protein to avoid the protein misfolded problem and loss of function.
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eGFP gene is the commonly used as a green signal fused with PVDQ to increase the stability.
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6x His tag is commonly used for protein purification
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T7 terminator is used for stoping the protein translation.
  
 
===Source===
 
===Source===

Latest revision as of 02:50, 5 October 2021


Elimination of AHL by E. bsuahlterminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

T7 promoter is a high efficiency element which can have a high protein level of LUXR inside the BL21 E. coli bacteria. High level of LUXR expressed will makes the BL21 more sensitive to AHL in the environment.

Lac operator is an inhibitory DNA element which can shut down the function of AHL sensing by inhibit the transcription of LuxR gene. Thus, the AHL release by the BL21 E. coli can not be detected when culturing at the initial steps.

pelB is a commonly used signaling peptide for locating the protein into periplasm.

pvdq is the most widely used gene in the quorum system. To block quorum system activation, we use the PVDQ protein to clean up all the AHL in the environment released by P. aeruginosa.

protein linker is a commonly used in the fused protein to avoid the protein misfolded problem and loss of function.

eGFP gene is the commonly used as a green signal fused with PVDQ to increase the stability.

6x His tag is commonly used for protein purification

T7 terminator is used for stoping the protein translation.

Source

Pseudomon asaeruginosa

References

Papaioannou,E.,Wahjudi,M.,Nadal-Jimenez,P.,Koch,G.,Setroikromo,R.,Quax,W.J.,2009. Quorum-quenching acylase reduces the virulence of Pseudomon asaeruginosa in a Caenorhabditis elegans infection model. Antimicrob. Agents Chemother. 53, 4891–4897.