Difference between revisions of "Part:BBa K3897000"
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<partinfo>BBa_K3897000 short</partinfo> | <partinfo>BBa_K3897000 short</partinfo> | ||
| − | + | HR2 Aptamer is an 86bp ssDNA strand that can be folded into 3D shapes to target Human Epidermal Growth Factor 2 (HER2) | |
<h1><b>Introduction</b></h1> | <h1><b>Introduction</b></h1> | ||
| − | + | <p> | |
| − | < | + | Human epidermal growth factor (HER2), belongs to the ErbB receptor tyrosine kinase family, involved in signal transduction pathways that regulate cell growth and differentiation. However, overexpression of HER2 can cause pathogenesis and progression of many carcinoma types. However, HER2 can also be used to treat these cancers. Current drugs like Herceptin bind HER2 through antibodies and inhibit its pathway, curing cancer. Some ADC drugs (Antibody-drug conjugates) also use HER2 as a target for binding, so drugs can be brought into proximity to the tumor cells. (e.g. T-DM1) |
| + | Aptamers are oligonucleotides that form unique 3D structures that can bind to practically anything, ranging from small molecules like ions, amanitin; to large proteins like HER2 in our case. | ||
| + | BBa_K3897000 is an aptamer, nicknamed HR2 aptamer, which specifically binds to HER2 ECD. HR2 aptamer is obtained from previous literature. It is synthesized through chemical synthesis, and its binding affinity for HER2 is tested through ELONA (An Enzyme-linked Oligonucleotide Assay). | ||
| + | </p> | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 02:28, 5 October 2021
HR2 Aptamer, for targeting HER2 receptors
HR2 Aptamer is an 86bp ssDNA strand that can be folded into 3D shapes to target Human Epidermal Growth Factor 2 (HER2)
Introduction
Human epidermal growth factor (HER2), belongs to the ErbB receptor tyrosine kinase family, involved in signal transduction pathways that regulate cell growth and differentiation. However, overexpression of HER2 can cause pathogenesis and progression of many carcinoma types. However, HER2 can also be used to treat these cancers. Current drugs like Herceptin bind HER2 through antibodies and inhibit its pathway, curing cancer. Some ADC drugs (Antibody-drug conjugates) also use HER2 as a target for binding, so drugs can be brought into proximity to the tumor cells. (e.g. T-DM1) Aptamers are oligonucleotides that form unique 3D structures that can bind to practically anything, ranging from small molecules like ions, amanitin; to large proteins like HER2 in our case. BBa_K3897000 is an aptamer, nicknamed HR2 aptamer, which specifically binds to HER2 ECD. HR2 aptamer is obtained from previous literature. It is synthesized through chemical synthesis, and its binding affinity for HER2 is tested through ELONA (An Enzyme-linked Oligonucleotide Assay).
Usage and Biology
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]