Difference between revisions of "Part:BBa K4040007"
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<partinfo>BBa_K4040007 short</partinfo>
 
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We use the transmembrane region of human CD8 molecule to build the CARs.
 
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===Usage and Biology===
 
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===Sequence and Features===
 
===Sequence and Features===
 
<partinfo>BBa_K4040007 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4040007 SequenceAndFeatures</partinfo>
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===Used for a CAR===
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The synthetic receptors were constructed to contain an scFv derived from an antibody recognizing the virus spike protein, CR3022, which has been reported to bind with the receptor-binding domain of the SARS-CoV-2 S glycoprotein with high affinity, and the CD8 transmembrane domain present in the aCD19 CAR for T cells. For the cytoplasmic domains, we used the common g subunit of Fc receptors (CARg), MEGF10 (CARMEGF10), MERTK(CARMERTK) and CD3z (CARz) in our study.
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==Background and detail description==
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===More details for CD8A===
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CD8A is an integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, it functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. CD8A interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, it recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of cytotoxic T-lymphocytes (CTLs). This mechanism enables CTLs to recognize and eliminate infected cells and tumor cells. In NK-cells, the presence of CD8A homodimers at the cell surface provides a survival mechanism allowing conjugation and lysis of multiple target cells. CD8A homodimer molecules also promote the survival and differentiation of activated lymphocytes into memory CD8 T-cells.
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[[File:T--NMU_China--cd8tm.png|thumb|center|300px|<b>Figure 1.</b> Structure of CD8A.]]
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Revision as of 08:16, 4 October 2021


CD8 Transmembrane Domain

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 22
  • 1000
    COMPATIBLE WITH RFC[1000]