Difference between revisions of "Part:BBa K4040006"
 
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<partinfo>BBa_K4040006 short</partinfo>
 
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===Sequence and Features===
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<partinfo>BBa_K4040006 SequenceAndFeatures</partinfo>
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===Usage and Biology===
 
The TEV protease is a highly site-specific cysteine protease that is found in the Tobacco Etch Virus(TEV).The optimum recognition site for this enzyme is the sequence Glu-Asn-Leu-Tyr-Phe-Gln-(Gly/Ser)(ENLYFQ(G/S)) and cleavage occurs between the Gln and Gly/Ser residues. The most commonly used sequence is ENLYFQG. The protease is used to cleave affinity tags from fusion proteins. The optimal temperature for cleavage is 30℃; also it can be used at temperatures as low as 4℃. It is recommended that the cleavage for each fusion protein be optimized by varying the amount of Recombinant Viral TEV Protease, reaction time, or incubation temperature. It can be removed by Ni2+ affinity resin.
 
The TEV protease is a highly site-specific cysteine protease that is found in the Tobacco Etch Virus(TEV).The optimum recognition site for this enzyme is the sequence Glu-Asn-Leu-Tyr-Phe-Gln-(Gly/Ser)(ENLYFQ(G/S)) and cleavage occurs between the Gln and Gly/Ser residues. The most commonly used sequence is ENLYFQG. The protease is used to cleave affinity tags from fusion proteins. The optimal temperature for cleavage is 30℃; also it can be used at temperatures as low as 4℃. It is recommended that the cleavage for each fusion protein be optimized by varying the amount of Recombinant Viral TEV Protease, reaction time, or incubation temperature. It can be removed by Ni2+ affinity resin.
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The usage in our project can be found in
 
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===Used for the synthetic receptor===
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The usage in our project and experimental results can be found in <b>gp130-TEV</b>(<partinfo>BBa_K4040020</partinfo>).
 
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===Usage and Biology===
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===Sequence and Features===
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<partinfo>BBa_K4040006 SequenceAndFeatures</partinfo>
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Latest revision as of 08:02, 4 October 2021


TEV protease

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 431
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage and Biology

The TEV protease is a highly site-specific cysteine protease that is found in the Tobacco Etch Virus(TEV).The optimum recognition site for this enzyme is the sequence Glu-Asn-Leu-Tyr-Phe-Gln-(Gly/Ser)(ENLYFQ(G/S)) and cleavage occurs between the Gln and Gly/Ser residues. The most commonly used sequence is ENLYFQG. The protease is used to cleave affinity tags from fusion proteins. The optimal temperature for cleavage is 30℃; also it can be used at temperatures as low as 4℃. It is recommended that the cleavage for each fusion protein be optimized by varying the amount of Recombinant Viral TEV Protease, reaction time, or incubation temperature. It can be removed by Ni2+ affinity resin.


Used for the synthetic receptor

The usage in our project and experimental results can be found in gp130-TEV(BBa_K4040020).