Difference between revisions of "Part:BBa K3804009"
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<partinfo>BBa_K3804009 short</partinfo> | <partinfo>BBa_K3804009 short</partinfo> | ||
− | We created this | + | We created this part with reference to <bbpart>BBa_K1024002</bbpart> in order to signal when Pseudomonas aeruginosa exists. The part is composed of pTEF, Flag, VP16, NLS, QscR, ADH1 terminator, pQsc, Cyc100mini, mCherry and CYC1terminator. We improved <bbpart>BBa_K1024002</bbpart> by adding ADH1 terminator and CYC1terminator and changed LuxR to QscR, pLux to pQsc. QscR is homolog of LusR. The QscR gene is constitutively expressed. On the other hands, activation of pQsc occurs only when sufficient amount of AHL exist in the environment. When pQsc was activated, mCherry is expressed and lets us know the existence of AHL. |
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K3804009 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3804009 SequenceAndFeatures</partinfo> | ||
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Latest revision as of 06:40, 3 October 2021
Reporter for quorum sensing systems in yeast with qscR
We created this part with reference to BBa_K1024002 in order to signal when Pseudomonas aeruginosa exists. The part is composed of pTEF, Flag, VP16, NLS, QscR, ADH1 terminator, pQsc, Cyc100mini, mCherry and CYC1terminator. We improved BBa_K1024002 by adding ADH1 terminator and CYC1terminator and changed LuxR to QscR, pLux to pQsc. QscR is homolog of LusR. The QscR gene is constitutively expressed. On the other hands, activation of pQsc occurs only when sufficient amount of AHL exist in the environment. When pQsc was activated, mCherry is expressed and lets us know the existence of AHL.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1673
Illegal PstI site found at 2036 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1673
Illegal PstI site found at 2036 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1673
Illegal XhoI site found at 1595
Illegal XhoI site found at 1646 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1673
Illegal PstI site found at 2036 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1673
Illegal PstI site found at 2036 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1811