Difference between revisions of "Part:BBa K3804008"
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<partinfo>BBa_K3804008 short</partinfo> | <partinfo>BBa_K3804008 short</partinfo> | ||
− | This part expresses LasI. LasI is a protein which | + | This part expresses LasI. LasI is a protein which synthesizes 3-oxo-C12-AHL. AHL is used for the quorum sensing system in Pseudomonas aeruginosa. It binds with LasR and forms a complex(AHL-LasR). Two AHL-LasR form a dimer and activate Las promoter. LasR has some homologues such as QscR and LuxR. In our project, We used this part in order to express AHL in yeast. |
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Latest revision as of 06:31, 3 October 2021
LasI
This part expresses LasI. LasI is a protein which synthesizes 3-oxo-C12-AHL. AHL is used for the quorum sensing system in Pseudomonas aeruginosa. It binds with LasR and forms a complex(AHL-LasR). Two AHL-LasR form a dimer and activate Las promoter. LasR has some homologues such as QscR and LuxR. In our project, We used this part in order to express AHL in yeast.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 270
- 1000COMPATIBLE WITH RFC[1000]