Difference between revisions of "BioScaffold Parts"
Line 37: Line 37:
 
|  ||α||[[Part:BBa_J70030]]||A BioScaffold Part (Protein Tail Remover/Protein Fusions)||PpiI||MabI, PacI
 
|  ||α||[[Part:BBa_J70030]]||A BioScaffold Part (Protein Tail Remover/Protein Fusions)||PpiI||MabI, PacI
 
|-
 
|-
−
|  ||α||[[Part:BBa_J70032]]||A Composite BioScaffold Part (Protein Fusions)||PpiI, PsrI||MabI
+
|  ||α||[[Part:BBa_J70032]]||A Composite BioScaffold Part (Protein Fusions)||PpiI, PsrI||MabI, (AscI and PaacI)
 
|-
 
|-
 
|  ||β||[[Part:BBa_J70034]]||A BioScaffold Part (for Library Vector Preparation)||AarI||XmaI
 
|  ||β||[[Part:BBa_J70034]]||A BioScaffold Part (for Library Vector Preparation)||AarI||XmaI

Revision as of 00:58, 28 December 2008

BioScaffold parts are BioBrick parts that contain restriction enzyme recognition sites that enable their full or partial removal from a BioBrick assembly.


The enzymes that facilitate the removal of BioScaffold parts are defined by what region of the part that they excise:
External Enzymes: Do not cut solely inside the BioBrick Part (extend to Prefix, Suffix, and/or Scar regions)
Internal Enzymes: Cut inside the BioBrick Part (between the Prefix, Suffix, and/or Scar regions)


The classes of α, β, and γ BioScaffold parts are defined for assemblies of the form:
<Prefix> part1 <Scar>BioScaffold part<Scar>part2<Suffix>
class α parts: specific enzymes cut outside of scars surrounding the BioScaffold part
class β parts: specific enzymes cut within scars surrounding BioScaffold part
class γ parts: no specific enzymes, internal enzymes cut within the BioScaffold part
mixed parts: contain properties of several classes, name classes involved


Currently, BioScaffold parts and their status must be manually added to the following table.
-?- Class Name Description External Enzymes Internal Enzymes
A α Part:BBa_J70010 A BioScaffold Part (for offset see Part Design page) PpiI MabI, PacI
A α Part:BBa_J70012 A BioScaffold Part (Protein Head Remover) PsrI MabI, AscI
α Part:BBa_J70030 A BioScaffold Part (Protein Tail Remover/Protein Fusions) PpiI MabI, PacI
α Part:BBa_J70032 A Composite BioScaffold Part (Protein Fusions) PpiI, PsrI MabI, (AscI and PaacI)
β Part:BBa_J70034 A BioScaffold Part (for Library Vector Preparation) AarI XmaI
β Part:BBa_J70036 A BioScaffold Part (for Library Insert Preparation) AarI XmaI


Current Plasmid Compatibility