Difference between revisions of "BioScaffold Parts"
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<center>Currently BioScaffold parts and their status must be manually added to the table. </center>
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<center>Currently, BioScaffold parts and their status must be manually added to the following table. </center>
 
{| border="1" cellspacing="0" align="center"
 
{| border="1" cellspacing="0" align="center"
 
!-?-!!Class!!Name!!Description!!External Enzymes!!Internal Enzymes
 
!-?-!!Class!!Name!!Description!!External Enzymes!!Internal Enzymes
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|A  ||&alpha;||[[Part:BBa_J70010]]||A BioScaffold Part (for offset see Part Design page)||PpiI||MabI, PacI
 
|A  ||&alpha;||[[Part:BBa_J70010]]||A BioScaffold Part (for offset see Part Design page)||PpiI||MabI, PacI
 
|-
 
|-
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|A  ||&alpha;||BBa_J70012||A BioScaffold Part (Protein Head Remover)||PsrI||MabI, AscI
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|A  ||&alpha;||[[Part:BBa_J70012]]||A BioScaffold Part (Protein Head Remover)||PsrI||MabI, AscI
 
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|-
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|  ||&alpha;||BBa_J70030||A BioScaffold Part (Protein Tail Remover/Protein Fusions)||PpiI||MabI, PacI
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|  ||&alpha;||[[Part:BBa_J70030]]||A BioScaffold Part (Protein Tail Remover/Protein Fusions)||PpiI||MabI, PacI
 
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|-
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|  ||&alpha;||BBa_J70032||A Composite BioScaffold Part (Protein Fusions)||PpiI, PsrI||MabI
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|  ||&alpha;||[[Part:BBa_J70032]]||A Composite BioScaffold Part (Protein Fusions)||PpiI, PsrI||MabI
 
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|  ||&beta;||BBa_J70034||A BioScaffold Part (for Library Vector Preparation)||AarI||XmaI
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|  ||&beta;||[[Part:BBa_J70034]]||A BioScaffold Part (for Library Vector Preparation)||AarI||XmaI
 
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|  ||&beta;||BBa_J70036||A BioScaffold Part (for Library Insert Preparation)||AarI||XmaI
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|  ||&beta;||[[Part:BBa_J70036]]||A BioScaffold Part (for Library Insert Preparation)||AarI||XmaI
 
|}
 
|}
  
  
 
==Current Plasmid Compatibility==
 
==Current Plasmid Compatibility==

Revision as of 00:57, 28 December 2008

BioScaffold parts are BioBrick parts that contain restriction enzyme recognition sites that enable their full or partial removal from a BioBrick assembly.


The enzymes that facilitate the removal of BioScaffold parts are defined by what region of the part that they excise:
External Enzymes: Do not cut solely inside the BioBrick Part (extend to Prefix, Suffix, and/or Scar regions)
Internal Enzymes: Cut inside the BioBrick Part (between the Prefix, Suffix, and/or Scar regions)


The classes of α, β, and γ BioScaffold parts are defined for assemblies of the form:
<Prefix> part1 <Scar>BioScaffold part<Scar>part2<Suffix>
class α parts: specific enzymes cut outside of scars surrounding the BioScaffold part
class β parts: specific enzymes cut within scars surrounding BioScaffold part
class γ parts: no specific enzymes, internal enzymes cut within the BioScaffold part
mixed parts: contain properties of several classes, name classes involved


Currently, BioScaffold parts and their status must be manually added to the following table.
-?- Class Name Description External Enzymes Internal Enzymes
A α Part:BBa_J70010 A BioScaffold Part (for offset see Part Design page) PpiI MabI, PacI
A α Part:BBa_J70012 A BioScaffold Part (Protein Head Remover) PsrI MabI, AscI
α Part:BBa_J70030 A BioScaffold Part (Protein Tail Remover/Protein Fusions) PpiI MabI, PacI
α Part:BBa_J70032 A Composite BioScaffold Part (Protein Fusions) PpiI, PsrI MabI
β Part:BBa_J70034 A BioScaffold Part (for Library Vector Preparation) AarI XmaI
β Part:BBa_J70036 A BioScaffold Part (for Library Insert Preparation) AarI XmaI


Current Plasmid Compatibility