Difference between revisions of "BioScaffold Parts"
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==Current Plasmid Compatibility==
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==Constitutive==
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If you're interested in constitutively expressing your protein of interest, use one of these plasmids.
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<parttable>constitutive_expression_plasmid</parttable>
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<!-- To include a new part in this table, make the part type "Plasmid" and add the categories "//plasmid/expression/constitutive //standard/biobrick " to the part under the Hard Information tab. -->
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If you're interested in constitutively expressing your protein of interest, use one of these plasmids.
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<parttable>constitutive_expression_plasmid</parttable>
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If you're interested in constitutively expressing your protein of interest, use one of these plasmids.
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<parttable>bioscaffold_plasmid</parttable>
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<!-- To include a new part in this table, make the part type "Plasmid" and add the categories
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"//plasmid/bioscaffold //standard/biobrick " to the part under the Hard Information tab. -->
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==Alpha==
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<parttable>plasmid_bioscaffold_alpha</parttable>
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<html>
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<style>
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#assembly_plasmid_table {margin-left:auto;margin-right:auto; border: 3px solid #444444;}
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#assembly_plasmid_table td {background-color:#eeeeee; padding: 5px; font-size: 12px;}
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#assembly_plasmid_table th {background-color: #aaaaaa;padding: 5px; font-size:14px;}
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</style>
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</html>
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<parttable>silver_protein_fusion_plasmid</parttable>
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<!-- To include a plasmid in this table, make the part type "Plasmid" and include the categories "//plasmid/proteinfusion //standard/silver" under the Hard Information tab of the part. -->
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<html>
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<style>
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#assembly_plasmid_table {margin-left:auto;margin-right:auto; border: 3px solid #444444;}
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#assembly_plasmid_table td {background-color:#eeeeee; padding: 5px; font-size: 12px;}
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#assembly_plasmid_table th {background-color: #aaaaaa;padding: 5px; font-size:14px;}
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</style>
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</html>
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<parttable>knight_protein_fusion_plasmid</parttable>
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<html>
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<style>
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#assembly_BioScaffold_table {margin-left:auto;margin-right:auto; border: 3px solid #444444;}
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#assembly_BioScaffold_table td {background-color:#eeeeee; padding: 5px; font-size: 12px;}
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#assembly_BioScaffold_table th {background-color: #aaaaaa;padding: 5px; font-size:14px;}
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</style>
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</html>
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<parttable>norville_protein_fusion_plasmid</parttable>
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<!-- To include a plasmid in this table, make the part type "Plasmid" and include the categories "//protein/bioscaffold //standard/norville" under the Hard Information tab of the part. -->
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Revision as of 00:50, 28 December 2008

BioScaffold parts are BioBrick parts that contain restriction enzyme recognition sites that enable their full or partial removal from a BioBrick assembly.


The enzymes that facilitate the removal of BioScaffold parts are defined by what region of the part that they excise:
External Enzymes: Do not cut solely inside the BioBrick Part (extend to Prefix, Suffix, and/or Scar regions)
Internal Enzymes: Cut inside the BioBrick Part (between the Prefix, Suffix, and/or Scar regions)


The classes of α, β, and γ BioScaffold parts are defined for assemblies of the form:
<Prefix> part1 <Scar>BioScaffold part<Scar>part2<Suffix>
class α parts: specific enzymes cut outside of scars surrounding the BioScaffold part
class β parts: specific enzymes cut within scars surrounding BioScaffold part
class γ parts: no specific enzymes, internal enzymes cut within the BioScaffold part
mixed parts: contain properties of several classes, name classes involved


Currently BioScaffold parts and their status must be manually added to the table.
-?- Class Name Description External Enzymes Internal Enzymes
A α BBa_J70010 A BioScaffold Part (for offset see Part Design page) PpiI MabI, PacI
A α BBa_J70012 A BioScaffold Part (Protein Head Remover) PsrI MabI, AscI
α BBa_J70030 A BioScaffold Part (Protein Tail Remover/Protein Fusions) PpiI MabI, PacI
α BBa_J70032 A Composite BioScaffold Part (Protein Fusions) PpiI, PsrI MabI
β BBa_J70034 A BioScaffold Part (for Library Vector Preparation) AarI XmaI
β BBa_J70036 A BioScaffold Part (for Library Insert Preparation) AarI XmaI


Current Plasmid Compatibility