Difference between revisions of "BioScaffold Parts"

Revision as of 00:48, 28 December 2008

BioScaffold parts are BioBrick parts that contain restriction enzyme recognition sites that enable their full or partial removal from a BioBrick assembly.

The enzymes that facilitate the removal of BioScaffold parts are defined by what region of the part that they excise:

External Enzymes: Do not cut solely inside the BioBrick Part (extend to Prefix, Suffix, and/or Scar regions)

Internal Enzymes: Cut inside the BioBrick Part (between the Prefix, Suffix, and/or Scar regions)

The classes of α, β, and γ BioScaffold parts are defined for assemblies of the form: <Prefix> part1 <Scar>BioScaffold part<Scar>part2<Suffix>

class α parts: specific enzymes cut outside of scars surrounding the BioScaffold part

class β parts: specific enzymes cut within scars surrounding BioScaffold part

class γ parts: no specific enzymes, internal enzymes cut within the BioScaffold part

mixed parts: contain properties of several classes, name classes involved

Currently BioScaffold parts and their status must be manually added to the table.

-?-	Class	Name	Description	External Enzymes	Internal Enzymes
A	α	BBa_J70010	A BioScaffold Part (for offset see Part Design page)	PpiI	MabI, PacI
A	α	BBa_J70012	A BioScaffold Part (Protein Head Remover)	PsrI	MabI, AscI
	α	BBa_J70030	A BioScaffold Part (Protein Tail Remover/Protein Fusions)	PpiI	MabI, PacI
	α	BBa_J70032	A Composite BioScaffold Part (Protein Fusions)	PpiI, PsrI	MabI
	β	BBa_J70034	A BioScaffold Part (for Library Vector Preparation)	AarI	XmaI
	β	BBa_J70036	A BioScaffold Part (for Library Insert Preparation)	AarI	XmaI

Constitutive

If you're interested in constitutively expressing your protein of interest, use one of these plasmids.

There are no parts for this table

If you're interested in constitutively expressing your protein of interest, use one of these plasmids.

There are no parts for this table

If you're interested in constitutively expressing your protein of interest, use one of these plasmids.

There are no parts for this table

Alpha

There are no parts for this table

@@ Line 27: / Line 27: @@
+<center>Currently BioScaffold parts and their status must be manually added to the table. </center>
 {| border="1" cellspacing="0" align="center"
 !-?-!!Class!!Name!!Description!!External Enzymes!!Internal Enzymes
@@ Line 41: / Line 41: @@
 |   ||&beta;||BBa_J70034||A BioScaffold Part (for Library Vector Preparation)||AarI||XmaI
 |-
-|   ||&beta:||BBa_J70036||A BioScaffold Part (for Library Insert Preparation)||AarI||XmaI
+|   ||&beta;||BBa_J70036||A BioScaffold Part (for Library Insert Preparation)||AarI||XmaI
 |}