Difference between revisions of "Part:BBa K3990013"
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Revision as of 16:47, 1 October 2021
T7+Laccase CotA [E34G;T87A;L182R] Expressing Construct
We will express the enzyme LaccaseCotA in BL21(DE3) E.coli to decompose the ethylene vinyl acetate copolymer(EVA).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 915
Illegal AgeI site found at 1222
Illegal AgeI site found at 1378 - 1000COMPATIBLE WITH RFC[1000]