Difference between revisions of "Part:BBa K3799007"

 
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<partinfo>BBa_K3799007 short</partinfo>
 
<partinfo>BBa_K3799007 short</partinfo>
  
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This part consist of the AgrC-AgrA quorum sensing signal transduction system of S.aureus(BBa_I746101)along with P2 promoter regulating GFP(BBa_K391000).The signal transduction system is regulated by constitutive promoter of B.subtilis Pveg(BBa_k143012).
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This part consist of the AgrC-AgrA quorum sensing signal transduction system of S.aureus regulated by Pveg promoter from B.subtilis(BBa_K3799010).The activity of this two component system inturn regulated P2 promoter which is studied using GFP expression(BBa_K391000)
  
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The AgrC-AgrA is a two component signal transduction system which senses the AIP-1 molecule secreted by S.aureus.AgrC encodes a transmembrane histidine kinase receptor which binds to exogenous AIP-1 secreted by other S.aureus which inturn phosphorylates intracellular AgrA which acts as a signalling molecule.It then binds to P2 promoter in the operon to activate it.
 
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The regulation of P2 promoter by the two component system is studied using GFP expression.Here,both pveg promoter and p2 promoter is aligned in a single direction.
 
  
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The regulation of P2 promoter by the two component system when both Pveg and P2 promoter are aligned in same direction is studied using this system.
 
<!-- Add more about the biology of this part here
 
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===Usage and Biology===
 
===Usage and Biology===

Revision as of 14:59, 1 October 2021


Unidirectional model of AIP-1 sensor of S aureus regulating GFP intensity using P2 promoter

This part consist of the AgrC-AgrA quorum sensing signal transduction system of S.aureus regulated by Pveg promoter from B.subtilis(BBa_K3799010).The activity of this two component system inturn regulated P2 promoter which is studied using GFP expression(BBa_K391000)


The regulation of P2 promoter by the two component system when both Pveg and P2 promoter are aligned in same direction is studied using this system. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 513
    Illegal BamHI site found at 65
    Illegal BamHI site found at 1397
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2953