Difference between revisions of "Plasmid backbones/Other standards"

Revision as of 23:44, 12 December 2008

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Part assembly	System operation	Protein expression	Assembly of protein fusions	Part measurement	Screening of part libraries	Building BioBrick vectors	DNA synthesis	Other standards	Archive

Or get some help on plasmid backbones.

For plasmid backbones that adhere to the Silver or Freiburg standards, please see plasmid backbones for protein fusions.

Berkeley assembly standard plasmid backbones

Researchers at UC Berkeley have developed a new assembly standard. See [http://openwetware.org/wiki/The_BioBricks_Foundation:Standards/Technical/Formats#The_Berkeley_.28BBb.29_Format the BioBricks Foundation wiki] for more details.

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Lim assembly standard plasmid backbones

Students in Wendell Lim's lab have developed a new assembly standard. See [http://2008.igem.org/Everything_you_ever_wanted_to_know_about_AarI the 2008 UCSF iGEM team wiki] for more details. Note these plasmid backbones are primarily intended for use when working with yeast.

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Sergio Peisajovich and Andrew Horowitz, from Wendell Lim's lab, developed several of the yeast plasmid backbones as an instructor of the 2008 UCSF iGEM team.

Plasmids used in DNA synthesis of BioBrick® parts

Most DNA synthesis companies do not currently use BioBrick® plasmid backbones to clone synthesized BioBrick® parts. Therefore, some parts in the Registry are available in non-standard plasmid backbones because they were constructed via direct DNA synthesis. Here is a list of plasmid backbones used by assorted DNA synthesis companies.

Other miscellaneous vectors

The Registry also have miscellaneous other plasmid backbones. These plasmid backbones have not undergone Registry curation, but we include them here for completeness.