Difference between revisions of "Part:BBa K3814023:Design"
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Self-dimerisation and hairpin formation metrics were calculated on PrimerSelect. Lowest values are as follows: | Self-dimerisation and hairpin formation metrics were calculated on PrimerSelect. Lowest values are as follows: | ||
| − | Self-dimerisation: -2.0kc/m (worst possible is - | + | Self-dimerisation: -2.0kc/m (worst possible is -88.1kc/m) |
Hairpin formation: 1.0kc/m | Hairpin formation: 1.0kc/m | ||
Revision as of 14:19, 1 October 2021
5' Homology Arm for Cluster 5
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Self-dimerisation and hairpin formation metrics were calculated on PrimerSelect. Lowest values are as follows:
Self-dimerisation: -2.0kc/m (worst possible is -88.1kc/m) Hairpin formation: 1.0kc/m
Source
iGEM USYD 2021