Difference between revisions of "Part:BBa K3843000:Design"

 
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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The GenBank sequence coded for a propeptide that also contained a signal sequence. We first identified the signal peptide using the bioinformatics server SignalP 5.0 (LINK), then we removed the signal peptide. We also removed the last 142 bp from the sequence, as these were annotated to not be present in the mature peptide by the authors of the GenBank submission (CITE). The resulting sequence gives rise to functional salmon trypsin, appropriate for use in a fusion protein.
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The GenBank sequence coded for a propeptide that also contained a signal sequence. We first codon optimized the sequence for Escherichia coli K12 and simultaneously removed illegal restriction sites for RFC[10]. Next, we identified the signal peptide using the bioinformatics server SignalP 5.0 (LINK), then we removed the signal peptide. We also removed the last 142 bp from the sequence, as these were annotated to not be present in the mature peptide by the authors of the GenBank submission (CITE). Finally, we added RFC[10]-compatible prefixes and suffixes. The resulting sequence gives rise to functional salmon trypsin, appropriate for use in a fusion protein.
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===Source===
 
===Source===

Revision as of 05:21, 1 October 2021


PEA-binding salmon trypsin (1UTM)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The GenBank sequence coded for a propeptide that also contained a signal sequence. We first codon optimized the sequence for Escherichia coli K12 and simultaneously removed illegal restriction sites for RFC[10]. Next, we identified the signal peptide using the bioinformatics server SignalP 5.0 (LINK), then we removed the signal peptide. We also removed the last 142 bp from the sequence, as these were annotated to not be present in the mature peptide by the authors of the GenBank submission (CITE). Finally, we added RFC[10]-compatible prefixes and suffixes. The resulting sequence gives rise to functional salmon trypsin, appropriate for use in a fusion protein.

Source

The sequence of this part was obtained from GenBank: https://www.ncbi.nlm.nih.gov/nuccore/X70071.

References