Difference between revisions of "Part:BBa K3794000:Design"
Line 9: Line 9:
 
Removed signal sequence from UniProt U5Y3S6. Codon optimised for expression in E.Coli K12  
 
Removed signal sequence from UniProt U5Y3S6. Codon optimised for expression in E.Coli K12  
  
−
Contains a 6xHis tag and TEV site for protein purification.
+
 
  
  

Revision as of 23:29, 30 September 2021


PVFP-5


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Removed signal sequence from UniProt U5Y3S6. Codon optimised for expression in E.Coli K12



Source

This part was synthesised by IDT. The DNA sequence was obtained from UniProtKB: U5Y3S6, and codon optimised for expression in E.Coli

References