Difference between revisions of "Part:BBa K3726000"
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The codified enzyme (NphT7) catalyzes the irreversible condensation-decarboxylation step of 1 molecule of acetyl-coA with Malonyl-coA to transform them into Acetoacetyl-coA and CO2.
 
The codified enzyme (NphT7) catalyzes the irreversible condensation-decarboxylation step of 1 molecule of acetyl-coA with Malonyl-coA to transform them into Acetoacetyl-coA and CO2.
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<b>Part Source</b>
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This Acetoacetyl CoA synthase has been found within the genome of Streptomyces sp. CL190.
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Uniprot reference: https://www.uniprot.org/uniprot/D7URV0
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<b>Design Considerations</b>
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This part corresponds with a codon optimized coding sequence of NphT7 enzyme.
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Codon optimization has been performed using DeNovo DNA Software, adjusting the codon usage for high expression in Synechococcus elongatus PCC7942.
  
  

Revision as of 15:00, 30 September 2021


CDS_NphT7

This part corresponds with the protein coding sequence for an Acetoacetyl CoA synthase.

The codified enzyme (NphT7) catalyzes the irreversible condensation-decarboxylation step of 1 molecule of acetyl-coA with Malonyl-coA to transform them into Acetoacetyl-coA and CO2.

Part Source

This Acetoacetyl CoA synthase has been found within the genome of Streptomyces sp. CL190.

Uniprot reference: https://www.uniprot.org/uniprot/D7URV0


Design Considerations

This part corresponds with a codon optimized coding sequence of NphT7 enzyme.

Codon optimization has been performed using DeNovo DNA Software, adjusting the codon usage for high expression in Synechococcus elongatus PCC7942.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 461
    Illegal PstI site found at 366
    Illegal PstI site found at 614
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 461
    Illegal PstI site found at 366
    Illegal PstI site found at 614
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 461
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 461
    Illegal PstI site found at 366
    Illegal PstI site found at 614
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 461
    Illegal PstI site found at 366
    Illegal PstI site found at 614
    Illegal NgoMIV site found at 105
    Illegal NgoMIV site found at 205
    Illegal NgoMIV site found at 511
    Illegal AgeI site found at 883
  • 1000
    COMPATIBLE WITH RFC[1000]