Difference between revisions of "Part:BBa K3804013:Design"

 
 
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===Design Notes===
 
===Design Notes===
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No.
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We added stop codon(taa) at the end of HBD3(BBa_K3351002). Thus, the length of the sequence is 742.
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===References===
 
===References===
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TS. Moller, J. Hay, MJ. Saxton, K. Bunting, EI. Petersen, S. Kjaerulff, et al., Human beta-defensin-2 production from S. cerevisiae using the repressible MET17 promoter, Microbial cell factories 16 (1) (2017) 11, https://doi.org/10.1186/s12934-017-0627-7.

Latest revision as of 12:56, 30 September 2021


HBD3_yeast _constitutive expression


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We added stop codon(taa) at the end of HBD3(BBa_K3351002). Thus, the length of the sequence is 742.


Source

The part consists of pCYC promoter, kozak, MFα1tag, HBD3 and ADH1 terminator.

References

TS. Moller, J. Hay, MJ. Saxton, K. Bunting, EI. Petersen, S. Kjaerulff, et al., Human beta-defensin-2 production from S. cerevisiae using the repressible MET17 promoter, Microbial cell factories 16 (1) (2017) 11, https://doi.org/10.1186/s12934-017-0627-7.