Difference between revisions of "Part:BBa K3934015"
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A 6xHis-tag is a sequence consisting of 6 histidines often used a protein tag for purification. The tag binds to certain metal ions (Ni2+, Co2+) which is useful for immobilized metal ion affinity chromatography (IMAC). | A 6xHis-tag is a sequence consisting of 6 histidines often used a protein tag for purification. The tag binds to certain metal ions (Ni2+, Co2+) which is useful for immobilized metal ion affinity chromatography (IMAC). | ||
| − | + | ===Sequence and Features=== | |
<partinfo>BBa_K3934015 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3934015 SequenceAndFeatures</partinfo> | ||
Revision as of 12:22, 30 September 2021
6xHis-Tag for protein purification
A 6xHis-tag is a sequence consisting of 6 histidines often used a protein tag for purification. The tag binds to certain metal ions (Ni2+, Co2+) which is useful for immobilized metal ion affinity chromatography (IMAC).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]