Difference between revisions of "Part:BBa K3739070"
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+ | __NOTOC__ | ||
+ | <partinfo>BBa_K3739070 short</partinfo> | ||
+ | Ag43 is a surface display protein and GFP can show the expression effciency of Ag43, Tevp can cut Ag43, leaving pelB as a signal peptide. | ||
+ | |||
+ | |||
+ | <!-- Add more about the biology of this part here | ||
+ | ===Usage and Biology=== | ||
+ | |||
+ | <!-- --> | ||
+ | <span class='h3bb'>Sequence and Features</span> | ||
+ | <partinfo>BBa_K3739070 SequenceAndFeatures</partinfo> | ||
+ | |||
+ | |||
+ | <!-- Uncomment this to enable Functional Parameter display | ||
+ | ===Functional Parameters=== | ||
+ | <partinfo>BBa_K3739070 parameters</partinfo> | ||
+ | <!-- --> |
Latest revision as of 17:59, 29 September 2021
J23100-B0030-pelB-Ag43-160N-his-GFP-B0010-J23100-B0030-pelB-Ag43-160N-Tevp-B0010
Ag43 is a surface display protein and GFP can show the expression effciency of Ag43, Tevp can cut Ag43, leaving pelB as a signal peptide.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 3590
Illegal NheI site found at 3613 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 118
Illegal NgoMIV site found at 1076
Illegal NgoMIV site found at 2300
Illegal NgoMIV site found at 2810
Illegal NgoMIV site found at 2831
Illegal NgoMIV site found at 3701
Illegal NgoMIV site found at 4539
Illegal AgeI site found at 1826
Illegal AgeI site found at 2570
Illegal AgeI site found at 2984
Illegal AgeI site found at 3226
Illegal AgeI site found at 5289
Illegal AgeI site found at 6033
Illegal AgeI site found at 6447 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 206