Difference between revisions of "Part:BBa K1149035"

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Proteinase K

Proteinase K is a member of serine-protease which is synthesized by the fungus Tritirachium album Limber [1] Encodes a peptidase from proteinase K family, breaks down polylactic acid

Proteinase K has the activity to degrade biofilm against several microorganisms including S. aureus, Listeria monocytogenes, Staphylococcus lugdunensis, Staphylococcus haemolyticus, Gardnerella vaginalis, Escherichia coli, Haemophilus influenzae, and Bdellovibrio bacteriovorus [2].Previously, high doses of Proteinase K (25–200 µg/mL) successfully inhibited biofilm formation and degraded preformed biofilm of H. pylori G27 after 24 hours, 37°C [3]. Proteinase K has two sites Ca 2+ binding. First binding site (Ca1) consist of Asp200 carboxylate and Pro175 peptide C = O in an apical, and Val177 peptide C = O and four water molecules in an equatorial position. Second binding site (Ca2) consists of the carboxylate of Asp260, the peptide C = 0 of Val16 and two water molecules[4]. Ca2+ ion gives protection against autolysis and increases thermal stability of proteinase K. Its activity reduces to 20% of original activity within 6 h after Ca 2+ is removed by gel filtration treatment with EDTA. Addition of Ca2+ after 6 h, just restores 28% of its original activity. The loss of Ca2+ ions causes a change in the conformational structure[4].Autolysis of Proteinase K without Ca 2+ ion happens after incubation >48 h. Removal of Ca2+ decreases the temperature stability of Proteinase K half enzymatic activity from 65°C in normal state to 46°C[4]. Concentration of 8M urea can decrease Proteinase K activity 65%. Furthermore Proteinase K also lost its activity when given SDS at concentration above 12.5% [4] . Autolysis does not occur within at least 48h when the concentration of Proteinase K is at or higher than 1.0 mg/ml, but when the concentration is at or below 0.01 mg/ml in aqueous solution, autolysis occurs slowly after 2h. This condition happens at pH 8 [5]. Optimum temperature of Proteinase K for maximum activity at 37°C and its activity remains constant until 55°C. 20% of methanol can reduces 50% activity of Proteinase K at optimum temperature[5]. Proteinase K shows 90% of original activity when given 10% methanol. But Proteinase K activity can be reduced to only 10% of original activity when 40% methanol is present [5]. Inhibition by chloromethyl ketone derivatives, Z-AlaAla-CK and Z-Ala-Phe-CK, just reached a maximum 50% of Proteinase K activity at 10 molar excess relative to Proteinase K. Even 25 fold excess synthetic inhibitors cannot increase levels of maximum inhibition activity of Proteinase K. This procedure is carried out in 50 mM Tris-HC1 (pH 8.0) contains 50% methanol [5].

Reference 1. EBELING W, HENNRICH N, KLOCKOW M, METZ H, ORTH H, LANG H. Proteinase K from Tritirachium album Limber. European Journal of Biochemistry. 1974;47(1):91-97. 2. Fleming D, Rumbaugh K. Approaches to Dispersing Medical Biofilms. Microorganisms. 2017;5(2):15. 3. Windham I, Servetas S, Whitmire J, Pletzer D, Hancock R, Merrell D. Helicobacter pylori Biofilm Formation Is Differentially Affected by Common Culture Conditions, and Proteins Play a Central Role in the Biofilm Matrix. Applied and Environmental Microbiology. 2018;84(14). 4. Bajorath J, Hinrichs W, Saenger W. The enzymatic activity of proteinase K is controlled by calcium. Eur J Biochem. 1988;176(2):441–7. 5. Bajorath J, Saenger W, Pal GP. Autolysis and inhibition of proteinase K, a subtilisin-related serine proteinase isolated from the fungus Tritirachium album Limber. Biochim Biophys Acta BBA - Protein Struct Mol Enzymol. 1988 Jan;954:176–82.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 787
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 134
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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--Proteinase K has the activity to degrade biofilm against several microorganisms including  S. aureus, Listeria monocytogenes, Staphylococcus lugdunensis, Staphylococcus haemolyticus, Gardnerella vaginalis, Escherichia coli, Haemophilus influenzae, and Bdellovibrio bacteriovorus [2]
+Proteinase K has the activity to degrade biofilm against several microorganisms including  S. aureus, Listeria monocytogenes, Staphylococcus lugdunensis, Staphylococcus haemolyticus, Gardnerella vaginalis, Escherichia coli, Haemophilus influenzae, and Bdellovibrio bacteriovorus [2].Previously, high doses of Proteinase K (25–200 µg/mL) successfully inhibited biofilm formation and degraded preformed biofilm of H. pylori G27 after 24 hours, 37°C [3]. Proteinase K has two sites Ca 2+ binding. First binding site (Ca1) consist of Asp200 carboxylate and Pro175 peptide C = O in an apical, and Val177 peptide C = O and four water molecules in an equatorial position. Second binding site (Ca2) consists of  the carboxylate of Asp260, the peptide C = 0 of Val16 and two water molecules[4]. Ca2+ ion gives protection against autolysis and increases thermal stability of proteinase K. Its activity reduces to 20% of original activity within 6 h after Ca 2+ is removed by gel filtration treatment with EDTA. Addition of Ca2+ after 6 h, just restores 28% of its original activity. The loss of Ca2+ ions causes a change in the conformational structure[4].Autolysis of Proteinase K without Ca 2+ ion happens after incubation >48 h. Removal of Ca2+ decreases the temperature stability of Proteinase K half enzymatic activity from 65°C in normal state to 46°C[4]. Concentration of 8M urea can decrease Proteinase K activity 65%. Furthermore Proteinase K also lost its activity when given SDS at concentration above 12.5%  [4] . Autolysis does not occur within at least 48h  when the concentration of Proteinase K is at or higher than 1.0 mg/ml, but when the concentration is at or below 0.01 mg/ml in aqueous solution, autolysis occurs slowly after 2h. This condition happens at pH 8 [5]. Optimum temperature of Proteinase K for maximum activity at 37°C and its activity remains constant until 55°C. 20% of methanol can reduces 50% activity of Proteinase K at optimum temperature[5]. Proteinase K shows 90% of original activity when given 10% methanol. But  Proteinase K activity can be reduced to only 10% of original activity when 40% methanol is present [5]. Inhibition by chloromethyl ketone derivatives, Z-AlaAla-CK and Z-Ala-Phe-CK, just reached a maximum 50% of Proteinase K activity at 10 molar excess relative to Proteinase K. Even 25 fold excess synthetic inhibitors cannot increase levels of maximum inhibition activity of Proteinase K. This procedure is carried out in 50 mM Tris-HC1 (pH 8.0) contains 50% methanol [5].
--Previously, high doses of Proteinase K (25–200 µg/mL) successfully inhibited biofilm formation and degraded preformed biofilm of H. pylori G27 after 24 hours, 37°C [3]
--Proteinase K has two sites Ca 2+ binding. First binding site (Ca1) consist of Asp200 carboxylate and Pro175 peptide C = O in an apical, and Val177 peptide C = O and four water molecules in an equatorial position. Second binding site (Ca2) consists of  the carboxylate of Asp260, the peptide C = 0 of Val16 and two water molecules[4].
--Ca2+ ion gives protection against autolysis and increases thermal stability of proteinase K. Its activity reduces to 20% of original activity within 6 h after Ca 2+ is removed by gel filtration treatment with EDTA. Addition of Ca2+ after 6 h, just restores 28% of its original activity. The loss of Ca2+ ions causes a change in the conformational structure[4].
--Autolysis of Proteinase K without Ca 2+ ion happens after incubation >48 h. Removal of Ca2+ decreases the temperature stability of Proteinase K half enzymatic activity from 65°C in normal state to 46°C[4].
--Concentration of 8M urea can decrease Proteinase K activity 65%. Furthermore Proteinase K also lost its activity when given SDS at concentration above 12.5%  [4]
--Autolysis does not occur within at least 48h  when the concentration of Proteinase K is at or higher than 1.0 mg/ml, but when the concentration is at or below 0.01 mg/ml in aqueous solution, autolysis occurs slowly after 2h. This condition happens at pH 8 [5]
--Optimum temperature of Proteinase K for maximum activity at 37°C and its activity remains constant until 55°C. 20% of methanol can reduces 50% activity of Proteinase K at optimum temperature[5]
--Proteinase K shows 90% of original activity when given 10% methanol. But  Proteinase K activity can be reduced to only 10% of original activity when 40% methanol is present [5].
--Inhibition by chloromethyl ketone derivatives, Z-AlaAla-CK and Z-Ala-Phe-CK, just reached a maximum 50% of Proteinase K activity at 10 molar excess relative to Proteinase K. Even 25 fold excess synthetic inhibitors cannot increase levels of maximum inhibition activity of Proteinase K. This procedure is carried out in 50 mM Tris-HC1 (pH 8.0) contains 50% methanol [5].
 Reference