Difference between revisions of "Part:BBa K3866017"

 
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<partinfo>BBa_K3866017 short</partinfo>
 
<partinfo>BBa_K3866017 short</partinfo>
  
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[[File:T--Thessaly--T5.MazF.TT.png|700px|thumb|none|<i><b>Fig.2:</b>The level A of MazF toxin with the partial T5 promoter and the double terminator in an alpha1R vector.</i>]]
  
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===Usage and Biology===
 
===Usage and Biology===
  
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This part includes the strong Partial T5 Promoter with the downstream operator Cuo. Also, it includes the toxin MazF that will be utilized to the toxin-antitoxin system and the double terminator.
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<span class='h3bb'>Sequence and Features</span>
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===Design Notes===
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The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in alpha2 vector  <bbpart>BBa_K3505009</bbpart> and has overhangs compatible for GoldenBraid cloning.
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===Sequence and Features===
 
<partinfo>BBa_K3866017 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3866017 SequenceAndFeatures</partinfo>
  
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===Source===
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Synthesized by Twist Biosciences.
  
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===References===
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===Functional Parameters===
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*Choi, Y. J., Morel, L., Le François, T., Bourque, D., Bourget, L., Groleau, D., Massie, B., & Míguez, C. B. (2010). Novel, versatile, and tightly regulated expression system for Escherichia coli strains. Applied and environmental microbiology, 76(15), 5058–5066. https://doi.org/10.1128/AEM.00413-10
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<partinfo>BBa_K3866017 parameters</partinfo>
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*Choi, W., Yamaguchi, Y., Park, J.-Y., Park, S.-H., Lee, H.-W., Lim, B.-K., … Park, J.-H. (2021). Functional Characterization of the mazEF Toxin-Antitoxin System in the Pathogenic Bacterium Agrobacterium tumefaciens. Microorganisms, 9(5), 1107. doi:10.3390/microorganisms9051107
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Latest revision as of 10:46, 29 September 2021


PArtial T5 - CuO - MazF - double terminator

Fig.2:The level A of MazF toxin with the partial T5 promoter and the double terminator in an alpha1R vector.

Usage and Biology

This part includes the strong Partial T5 Promoter with the downstream operator Cuo. Also, it includes the toxin MazF that will be utilized to the toxin-antitoxin system and the double terminator.



Design Notes

The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in alpha2 vector BBa_K3505009 and has overhangs compatible for GoldenBraid cloning.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Source

Synthesized by Twist Biosciences.

References

  • Choi, Y. J., Morel, L., Le François, T., Bourque, D., Bourget, L., Groleau, D., Massie, B., & Míguez, C. B. (2010). Novel, versatile, and tightly regulated expression system for Escherichia coli strains. Applied and environmental microbiology, 76(15), 5058–5066. https://doi.org/10.1128/AEM.00413-10
  • Choi, W., Yamaguchi, Y., Park, J.-Y., Park, S.-H., Lee, H.-W., Lim, B.-K., … Park, J.-H. (2021). Functional Characterization of the mazEF Toxin-Antitoxin System in the Pathogenic Bacterium Agrobacterium tumefaciens. Microorganisms, 9(5), 1107. doi:10.3390/microorganisms9051107