Difference between revisions of "Part:BBa K3804009:Design"
(2 intermediate revisions by the same user not shown) | |||
Line 7: | Line 7: | ||
===Design Notes=== | ===Design Notes=== | ||
− | Basically, the part is same to <bbpart>BBa_K1024002</bbpart>. When we describe the part, We used different parts which is similar to the | + | Basically, the part is same to <bbpart>BBa_K1024002</bbpart>. When we describe the part, We used different parts which is similar to the components of <bbpart>BBa_K1024002</bbpart> for convenience. The real sequence which we used is mutant of <bbpart>BBa_K3804010</bbpart> which was changed LasR to QscR and pLas to pQsc. |
− | + | ||
===Source=== | ===Source=== | ||
− | pTEF( <bbpart>BBa_K1024002</bbpart>), tVP16( <bbpart>BBa_K1024002</bbpart>), NLS( <bbpart>BBa_K1024002</bbpart>), QscR(<bbpart>BBa_K3804002</bbpart>), ADH1 terminator(<bbpart>BBa_J63002</bbpart>), pQsc(<bbpart>BBa_K3804003</bbpart>), | + | pTEF( <bbpart>BBa_K1024002</bbpart>), tVP16( <bbpart>BBa_K1024002</bbpart>), NLS( <bbpart>BBa_K1024002</bbpart>), QscR(<bbpart>BBa_K3804002</bbpart>), ADH1 terminator(<bbpart>BBa_J63002</bbpart>), pQsc(<bbpart>BBa_K3804003</bbpart>), CYC100mini( <bbpart>BBa_K1024002</bbpart>), mCherry( <bbpart>BBa_K1024002</bbpart>), CYC1 terminator(<bbpart>BBa_K1462070</bbpart>) |
===References=== | ===References=== |
Latest revision as of 10:25, 29 September 2021
Reporter for quorum sensing systems in yeast with qscR
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1673
Illegal PstI site found at 2036 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1673
Illegal PstI site found at 2036 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1673
Illegal XhoI site found at 1595
Illegal XhoI site found at 1646 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1673
Illegal PstI site found at 2036 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1673
Illegal PstI site found at 2036 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1811
Design Notes
Basically, the part is same to BBa_K1024002. When we describe the part, We used different parts which is similar to the components of BBa_K1024002 for convenience. The real sequence which we used is mutant of BBa_K3804010 which was changed LasR to QscR and pLas to pQsc.
Source
pTEF( BBa_K1024002), tVP16( BBa_K1024002), NLS( BBa_K1024002), QscR(BBa_K3804002), ADH1 terminator(BBa_J63002), pQsc(BBa_K3804003), CYC100mini( BBa_K1024002), mCherry( BBa_K1024002), CYC1 terminator(BBa_K1462070)