Difference between revisions of "Part:BBa K3804002"
 
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<partinfo>BBa_K3804002 short</partinfo>
 
<partinfo>BBa_K3804002 short</partinfo>
  
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This is a Qsc receptor which is designed to use in S.Cerevisiae. QscR bind with AHL and form AHL-QscR complex. Two AHL-QscR complexes form　a dimer and activate pQscR.  
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This is a Qsc receptor which is designed to be used in S.Cerevisiae. QscR, homolog of lasR, binds with AHL and forms the AHL-QscR complex. Two AHL-QscR complexes form a dimer and activate pQsc. There are some receptors which bind with AHL, such as luxR and lasR. Originally, pQsc exists in Pseudomonas aeruginosa and is used for quorum sensing. In Pseudomonas aeruginosa, AHL expresses constitutively and the amount of AHL is related to the density of Pseudomonas aeruginosa. We create this part in order to detect Pseudomonas aeruginosa by using the part in yeast.
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Originally, pQscR exists in Pseudomonas aeruginosa and is used for quorum sensing. In Pseudomonas aeruginosa, AHL is expressing constitutively and the amount of AHL is related to the density of Pseudomonas aeruginosa.  
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We create this part in order to detect Pseudomonas aeruginosa by using the part in yeast.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 09:05, 29 September 2021


Qsc receptor

This is a Qsc receptor which is designed to be used in S.Cerevisiae. QscR, homolog of lasR, binds with AHL and forms the AHL-QscR complex. Two AHL-QscR complexes form a dimer and activate pQsc. There are some receptors which bind with AHL, such as luxR and lasR. Originally, pQsc exists in Pseudomonas aeruginosa and is used for quorum sensing. In Pseudomonas aeruginosa, AHL expresses constitutively and the amount of AHL is related to the density of Pseudomonas aeruginosa. We create this part in order to detect Pseudomonas aeruginosa by using the part in yeast.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 103
    Illegal PstI site found at 466
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 103
    Illegal PstI site found at 466
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 103
    Illegal XhoI site found at 25
    Illegal XhoI site found at 76
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 103
    Illegal PstI site found at 466
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 103
    Illegal PstI site found at 466
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 241