Difference between revisions of "Part:BBa K3804009:Design"
Line 12: Line 12:
 
===Source===
 
===Source===
  
−
pTEF( <bbpart>BBa_K1024002</bbpart>), tVP16( <bbpart>BBa_K1024002</bbpart>), NLS( <bbpart>BBa_K1024002</bbpart>), QscR(<bbpart>BBa_K3804002</bbpart>), ADH1 terminator(<bbpart>BBa_J63002</bbpart>), pQsc(<bbpart>BBa_K3804003</bbpart>)
+
pTEF( <bbpart>BBa_K1024002</bbpart>), tVP16( <bbpart>BBa_K1024002</bbpart>), NLS( <bbpart>BBa_K1024002</bbpart>), QscR(<bbpart>BBa_K3804002</bbpart>), ADH1 terminator(<bbpart>BBa_J63002</bbpart>), pQsc(<bbpart>BBa_K3804003</bbpart>), cyc100mini( <bbpart>BBa_K1024002</bbpart>), mCherry( <bbpart>BBa_K1024002</bbpart>), CYC1 terminator(<bbpart>BBa_K1462070</bbpart>)
−
cyc100mini( <bbpart>BBa_K1024002</bbpart>), mCherry( <bbpart>BBa_K1024002</bbpart>), CYC1 terminator(<bbpart>BBa_K1462070</bbpart>)
+
  
 
===References===
 
===References===

Revision as of 07:46, 29 September 2021


Reporter for quorum sensing systems in yeast with qscR


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1673
    Illegal PstI site found at 2036
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1673
    Illegal PstI site found at 2036
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1673
    Illegal XhoI site found at 1595
    Illegal XhoI site found at 1646
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1673
    Illegal PstI site found at 2036
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1673
    Illegal PstI site found at 2036
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1811


Design Notes

Basically, the part is same to BBa_K1024002. When we describe the part, We used different parts which is similar to the component of BBa_K1024002 for convenience. However, the real sequence which we used is different from that. Therefore, please check the sequence part in main page carefully.


Source

pTEF( BBa_K1024002), tVP16( BBa_K1024002), NLS( BBa_K1024002), QscR(BBa_K3804002), ADH1 terminator(BBa_J63002), pQsc(BBa_K3804003), cyc100mini( BBa_K1024002), mCherry( BBa_K1024002), CYC1 terminator(BBa_K1462070)

References