Difference between revisions of "Part:BBa K3788021:Design"

 
Line 13: Line 13:
 
===Source===
 
===Source===
  
−
colA sequence was given by LISM laboratory from CNRS.
+
Sequence was given by LISM laboratory from CNRS, RFP and GFP coding sequence was taken corresponding to Part:BBa_E1010 and Part:BBa_E0040
  
  
  
 
===References===
 
===References===

Revision as of 20:13, 28 September 2021


Expression of GFP and of the timer device fused to the RFP protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1528
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1848
    Illegal BamHI site found at 1521
    Illegal XhoI site found at 1397
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1597
    Illegal AgeI site found at 2849
    Illegal AgeI site found at 2961
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 867
    Illegal SapI.rc site found at 1351


Design Notes

-


Source

Sequence was given by LISM laboratory from CNRS, RFP and GFP coding sequence was taken corresponding to Part:BBa_E1010 and Part:BBa_E0040


References