Difference between revisions of "Part:BBa K3788020:Design"

 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
XbaI restriction site was delated
 
  
  
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===Source===
 
===Source===
  
colA sequence was given by LISM laboratory from CNRS. GFP coding sequence was taken corresponding to Part:BBa_E0040
+
Sequences was given by LISM laboratory from CNRS. RFP coding sequence was taken corresponding to Part:BBa_E1010
  
  
  
 
===References===
 
===References===

Revision as of 20:08, 28 September 2021


Expression of the timer device fused to the RFP protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 808
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1128
    Illegal BamHI site found at 801
    Illegal XhoI site found at 677
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 877
    Illegal AgeI site found at 2129
    Illegal AgeI site found at 2241
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 631


Design Notes

Source

Sequences was given by LISM laboratory from CNRS. RFP coding sequence was taken corresponding to Part:BBa_E1010


References