Difference between revisions of "Part:BBa K3788019"

Revision as of 20:01, 28 September 2021

Expression of GFP and of the timer device under the control of the LexA-controlled promoter

Induced by the SOS response (LexA dependent) upon DNA damages (UV, mitomycin…). This promoter allow the expression of the operon caa and cal. caa gene is coding for Colicin A (ColA) a toxin toxin produced by E. coli strains, and active against other non-immune E.coli strains. In this contruction GFP permit to understand the mechanism, and ColA isn't able to have his toxin activity. To protect themselfs, E. coli have an imumnity protein coded by cai gene (-). cal gene is codding for a lysis protein, when this protein is produce ColA can be delivered in the extracellular medium after the E. coli cells lysis. The regulation is complex and involves many regulatory elements: terminator (+) and (-), promoters, antisense coding sequence, etc.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1528
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1848
Illegal BamHI site found at 1521
Illegal XhoI site found at 1397
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 1597
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 867
Illegal SapI.rc site found at 1351

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3788019 short</partinfo>
-Promotor induced by SOS signal. This promotor is involved in the colA regulation system of E. coli.
-Colicine are toxins produce by E. coli, among her the Colicine A (ColA).
+Induced by the SOS response (LexA dependent) upon DNA damages (UV, mitomycin…). This promoter allow the expression of the operon caa and cal. caa gene is coding for Colicin A (ColA) a toxin toxin produced by E. coli strains, and active against other non-immune E.coli strains. In this contruction GFP permit to understand the mechanism, and ColA isn't able to have his toxin activity. To protect themselfs, E. coli have an imumnity protein coded by cai gene (-). cal gene is codding for a lysis protein, when this protein is produce ColA can be delivered in the extracellular medium after the E. coli cells lysis. The regulation is complex and involves many regulatory elements: terminator (+) and (-), promoters, antisense coding sequence, etc.
-Cai is a immunity protein against Colicine A (COlA). ColA is toxic by inserted into the Escherichia coli inner membrane, Cai will inhibited this processus.
-Cal coding by cal gene cal is a polypeptide needed for the release of colicin A in the ColA system. In this contruction RFP permit to understand the mechanism, and Cail isn't able to have his lysis activity.
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