Difference between revisions of "Part:BBa K3757007"

Revision as of 14:43, 28 September 2021

35S Oak1

Gene encoding the cyclotide precursor Oak1 (BBa_K3757001), C-terminally tagged with a c-myc tag (BBa_K3757008). Regulated by the eukaryotic constitutive CaMV 35S promoter (BBa_K788000) and the 35S terminator polyadenylation signal (BBa_K1159307). In one Golden Gate cloning step, a sequence encoding a peptide can be cloned into the Oak1 precursor. This gene can then be co-expressed with CtAEP1 in Nicotiana benthamiana leading to expression of the peptide in cyclized form.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1468
Illegal BamHI site found at 1858
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 66

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 <partinfo>BBa_K3757007 short</partinfo>
-Gene encoding the cyclotide precursor Oak1 (BBa_K3757001), C-terminally tagged with a c-myc tag (BBa_K3757007). Regulated by the eukaryotic constitutive CaMV 35S promoter (BBa_K788000) and the 35S terminator polyadenylation signal (BBa_K1159307). In one Golden Gate cloning step, a sequence encoding a peptide can be cloned into the Oak1 precursor. This gene can then be co-expressed with CtAEP1 in Nicotiana benthamiana leading to expression of the peptide in cyclized form.
+Gene encoding the cyclotide precursor Oak1 (BBa_K3757001), C-terminally tagged with a c-myc tag (BBa_K3757008). Regulated by the eukaryotic constitutive CaMV 35S promoter (BBa_K788000) and the 35S terminator polyadenylation signal (BBa_K1159307). In one Golden Gate cloning step, a sequence encoding a peptide can be cloned into the Oak1 precursor. This gene can then be co-expressed with CtAEP1 in Nicotiana benthamiana leading to expression of the peptide in cyclized form.
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