Difference between revisions of "Part:BBa K3832008"
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| − | This Part is the Induced expression circuit of CDS aroG (Mutant s211f) [https://parts.igem.org/Part:BBa_K3832000(BBa_K3832000)].It contains lacUV5 promoter and a whole expression unit of lacI protein, thus aroG-s211f can be induced by IPTG. | + | This Part is the Induced expression circuit of CDS aroG (Mutant s211f) [https://parts.igem.org/Part:BBa_K3832000 (BBa_K3832000)].It contains lacUV5 promoter and a whole expression unit of lacI protein, thus aroG-s211f can be induced by IPTG. |
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Revision as of 06:35, 27 September 2021
Induced expression circuit of aroG (Mutant s211f)
This Part is the Induced expression circuit of CDS aroG (Mutant s211f) (BBa_K3832000).It contains lacUV5 promoter and a whole expression unit of lacI protein, thus aroG-s211f can be induced by IPTG.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 76
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 2469
Illegal NheI site found at 2492 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1034
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 76
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 76
- 1000COMPATIBLE WITH RFC[1000]