Difference between revisions of "Part:BBa K3715059:Design"
| Line 7: | Line 7: | ||
===Design Notes=== | ===Design Notes=== | ||
| − | + | This part is codon optimized in E. coli BL21. | |
| Line 13: | Line 13: | ||
===Source=== | ===Source=== | ||
| − | + | NCBI | |
| − | + | ||
===References=== | ===References=== | ||
Latest revision as of 15:56, 26 September 2021
T7 promoter+RBS+ His+Linker f+SUMO+Linker d+PETase_MT1+Linker e+T7 terminator
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 296
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 296
Illegal NheI site found at 73 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 296
Illegal BglII site found at 185
Illegal BamHI site found at 1285
Illegal XhoI site found at 384
Illegal XhoI site found at 1271 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 296
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 296
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part is codon optimized in E. coli BL21.
Source
NCBI