Difference between revisions of "Part:BBa K3832001:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | This part can be directly linked to the vector plasmid. No extra Promoter and Repressor gene(lacI) are required. | + | This part can be directly linked to the vector plasmid. No extra Promoter and Repressor gene(lacI) are required. |
| − | + | ||
| + | The following subparts are included in order： | ||
| + | lacUV5 promoter， lac operator， BBa_B0034(RBS), aroG-s211f(CDS), T7 terminator | ||
| + | T7Te terminator, rrnB T1 terminator, lacI(CDS), eSD, BBaJ23100(promoter) | ||
===Source=== | ===Source=== | ||
Revision as of 06:57, 26 September 2021
Induced expression circuit of aroG (Mutant s211f)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 2554
Illegal NheI site found at 2577 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2541
Illegal XhoI site found at 1146 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 453
Design Notes
This part can be directly linked to the vector plasmid. No extra Promoter and Repressor gene(lacI) are required.
The following subparts are included in order：
lacUV5 promoter， lac operator， BBa_B0034(RBS), aroG-s211f(CDS), T7 terminator T7Te terminator, rrnB T1 terminator, lacI(CDS), eSD, BBaJ23100(promoter)
Source
The sequence of the parts used in the device are gained from NCBI database, except the sequence of BBa_B0034 is gained from iGEM parts collections. Partial of the subparts are de-novo synthesized. All the subparts are assembled into this device by using GoldenGate assembly.