Difference between revisions of "Part:BBa K3866011"

Revision as of 17:25, 25 September 2021

MazF toxin GB compatible with B3-B5

MazF toxin is used in the toxin-antitoxin system with mazE anti-toxin.This is a toxin-antitoxin system type II, widely used at most labs. It binds to the MazF endoribonuclease toxin and neutralizes its endoribonuclease activity

Fig.2:J23115 with RBS

Usage and Biology

The MazEF system is an extensively studied type II TA module. MazF homologs are well known as ribosome-independent toxins and have sequence-specific endoribonucleases that can cleave mRNA sequences in a ribosome-independent manner.

Design Notes

The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in pUPD2 BBa_K3505007 and has overhangs compatible for GoldenBraid cloning. The Promoter has position B3-B5.

Fig.1:The overhangs of this part in the GoldenBraid Grammar

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Source

Synthesized by Twist Biosciences.

References

i

@@ Line 16: / Line 16: @@
 ===Design Notes===
-The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in pUPD2 BBa_K3505007 and has overhangs compatible for GoldenBraid cloning. The Promoter has position B3-B5.
+The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in pUPD2 <bbpart>BBa_K3505007</bbpart> and has overhangs compatible for GoldenBraid cloning. The Promoter has position B3-B5.
 [[File:T--Thessaly--GB-GGAG-AATG.jpeg|700px|thumb|none|<i><b>Fig.1:</b>The overhangs of this part in the GoldenBraid Grammar</i>]]