Difference between revisions of "Part:BBa K3866002"
 
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<partinfo>BBa_K3866002 short</partinfo>
 
<partinfo>BBa_K3866002 short</partinfo>
  
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    [[Image:T--Thessaly--acksnap.png|900px|thumb|none|<I><b>Figure 1.</b> The level 0 module : pupd2- ackA(illustration from SnapGene)</i>]]
  
 
===Usage and Biology===
 
===Usage and Biology===
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Catalyzes the formation of acetyl phosphate from acetate and ATP. Can also catalyze the reverse reaction.  
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AckA catalyzes the formation of acetyl phosphate from acetate and ATP. Can also catalyze the reverse reaction.
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It is utilized from our system for the reverse reaction in order to produce Acetate.
  
 
===Design Notes===
 
===Design Notes===
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<p style="text-align: center;">
 
<p style="text-align: center;">
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     [[Image: T--Thessaly--GB-AATG-GCTT.jpeg|900px|thumb|none|<i><b>Figure 1.</b>The overhangs of this part in the GoldenBraid Grammar.</i>]]
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     [[Image: T--Thessaly--GB-AATG-GCTT.jpeg|900px|thumb|none|<i><b>Figure 2.</b>The overhangs of this part in the GoldenBraid Grammar.</i>]]
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===Verification of Cloning===
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[[File:T--Thessaly--laci.png|700px|thumb|none|<i><b>Fig.3:</b>(U=Uncut C=Cut) Restriction Digestion of LacI with EcoRI + SlaI, Expected bands : 3189 +2300 + 892 bp, Positive Result:C2</i>]]
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===Source===
 
===Source===
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===Sequence and Features===
 
===Sequence and Features===
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<partinfo>BBa_K3866002 SequenceAndFeatures</partinfo>   9/6/2021
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<partinfo>BBa_K3866002 SequenceAndFeatures</partinfo>
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===References===
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Dittrich, C. R., Bennett, G. N., & San, K. Y. (2005). Characterization of the acetate-producing pathways in Escherichia coli. Biotechnology progress, 21(4), 1062–1067. https://doi.org/10.1021/bp050073s

Latest revision as of 14:22, 25 September 2021



ackA-Acetate kinase GB compatible with B3-B5


Figure 1. The level 0 module : pupd2- ackA(illustration from SnapGene)

Usage and Biology

AckA catalyzes the formation of acetyl phosphate from acetate and ATP. Can also catalyze the reverse reaction. It is utilized from our system for the reverse reaction in order to produce Acetate.

Design Notes

The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is presents in pUPD2 and has overhangs compatible for Golden Braid cloning. The CDS has position B3-B5.


Figure 2.The overhangs of this part in the GoldenBraid Grammar.

Source

Synthesized by Twist Biosciences.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

Dittrich, C. R., Bennett, G. N., & San, K. Y. (2005). Characterization of the acetate-producing pathways in Escherichia coli. Biotechnology progress, 21(4), 1062–1067. https://doi.org/10.1021/bp050073s